Protocols
For the best possible results, Cell Signaling Technology (CST) strongly recommends using our optimized application-specific protocols for each product. These protocols are the result of extensive in-house validation performed at CST and ensure accurate and reproducible results.
Product specific protocols will be linked from matching product web pages.
Western Blotting (Immunoblot)
- Western Immunoblotting Protocol (Western)
- Simple Western Protocol
- Western Immunoblotting (Fluorescent) Protocol (Fluorescent Western)
Immunoprecipitation
- Immunoprecipitation Protocol / (For Native Protein) (IP / Native Protein)
- Immunoprecipitation Protocol Utilizing Magnetic Separation / (For Analysis By Western Immunoblotting) (IP Magnetic)
Immunohistochemistry
- Immunohistochemistry Protocol (Paraffin) for Chimeric Antibodies
- Immunohistochemistry Protocol (Paraffin) for Direct Conjugates
- Immunohistochemistry Protocol (Paraffin) for SignalStain® Boost Detection Reagent (IHC / Paraffin)
- Immunohistochemistry Protocol (Frozen) for SignalStain® Boost Detection Reagent (IHC / Frozen)
- Fluorescent Multiplex Immunohistochemistry Protocol (mIHC/Paraffin)
- Immunohistochemistry Leica® BOND™ Protocol
Immunofluorescence
- Immunofluorescence Protocol for Cell-based Imaging (Immunocytochemistry)
- Immunofluorescence Protocol for Frozen Tissue (IF-F)
Flow Cytometry
- Flow Cytometry Methanol Permeabilization Protocol
- Flow Cytometry Triton™ X-100 Permeabilization Protocol
- Flow Cytometry FoxP3/Transcription Factor Fixation and Permeabilization Protocol
- Flow Cytometry Live Cell Protocol
Chromatin Immunoprecipitation
- SimpleChIP® Chromatin Immunoprecipitation Protocol (Agarose Beads) (ChIP Agarose)
- SimpleChIP® Chromatin Immunoprecipitation Protocol (Magnetic Beads) (ChIP Magnetic)
- SimpleChIP® Plus Chromatin Immunoprecipitation Protocol (Agarose Beads) (ChIP Agarose)
- SimpleChIP® Plus Chromatin Immunoprecipitation Protocol (Magnetic Beads) (ChIP Magnetic)
- SimpleChIP® Plus Sonication Chromatin Immunoprecipitation Protocol (Magnetic Beads) (ChIP Magnetic)
- DNA Library Prep Kit for Illumina® Protocol (ChIP-seq, CUT&RUN)
- Multiplex Oligos for Illumina® Protocol (Single Index Primers) (ChIP-seq, CUT&RUN)
- Multiplex Oligos for Illumina® Protocol (Dual Index Primers) (ChIP-seq, CUT&RUN)
CUT&RUN
- CUT&RUN Protocol
- CUT&RUN Assay Kit (with Drosophila Spike-In Control) Protocol
- Digestion of CUT&RUN Input DNA by MNase
- DNA Library Prep Kit for Illumina® Protocol (ChIP-seq, CUT&RUN)
- Multiplex Oligos for Illumina® Protocol (Single Index Primers) (ChIP-seq, CUT&RUN)
- Multiplex Oligos for Illumina® Protocol (Dual Index Primers) (ChIP-seq, CUT&RUN)
CUT&Tag
- CUT&Tag Protocol
- CUT&Tag Dual Index Primers and PCR Master Mix
- Drosophila Spike-In Control Kit for CUT&Tag (Rabbit) Protocol
- Drosophila Spike-In Control Kit for CUT&Tag (Mouse) Protocol
Sandwich Immunoassays (ELISA)
- PathScan® Sandwich ELISA Protocol (Colorimetric)
- PathScan® Rapid Sandwich ELISA Protocol (Colorimetric)
- PathScan® Sandwich ELISA Protocol (Chemiluminescent)
- PathScan® Rapid Sandwich ELISA Protocol (Chemiluminescent)
- FastScan® Sandwich ELISA Protocol
- PathScan® Sandwich ELISA Antibody Pair Protocol
- ELISA-Peptide Assay Protocol
- HRP Chemiluminescence ELISA-P
SignalStar Multiplex IHC
- SignalStar™ Multiplex Immunohistochemistry Assay Manual
- SignalStar™ Multiplex Immunohistochemistry Assay for Use on BOND RX Fully Automated Research Stainer by Leica Biosystems
- SignalStar™ Secondary Antibody Kit for Use Manually in SignalStar™ Multiplex Immunohistochemistry
- SignalStar™ Secondary Antibody Kit for Use on BOND RX Fully Automated Research Stainer by Leica Biosystems in SignalStar™ Multiplex Immunohistochemistry