Are the bands detected by your MT1-MMP (D1E4) Rabbit mAb #13130 the precursor (62 kDa) and active (50 kDa) forms?

Our MT1-MMP (D1E4) Rabbit mAb #13130 was produced with an antigenic peptide corresponding to residues surrounding Met293 of human MT1-MMP protein (see UniProt ID #P50281; https://www.uniprot.org/uniprot/P50281). Therefore, the antibody recognizes both the full-length MT1-MMP zymogen and the proteolytically processed, active enzyme (residues 112 to 582). The 62 kDa band represents the inactive zymogen that includes the inhibitory prodomain. MT1-MMP is activated by cleavage between Arg111 and Tyr112 to produce a mature/active enzyme [see Golubkov V.S. et al. (2007) J Biol Chem 282, 36283-91 (PMID: 17938169; https://www.ncbi.nlm.nih.gov/pubmed/17938169)] that migrates at approximately 50 kDa. We have not performed any direct testing to confirm that the 50 kDa band is definitively the cleaved form, but it is very likely the case based on the literature, expression levels, and epitope location.

Last updated: March 6, 2023