Technical Support Articles
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Why is there a discrepancy in the molecular weight of my target when analyzed by traditional western blot and Simple Western™?
The molecular weight reported by Simple Wesetern™ instruments like Jess™ is an "apparent" molecular weight as opposed to an "exact" molecular weight. The molecu...
What controls should I include when performing a Simple Western™ experiment?
In addition to a known positive control sample, we recommend the following negative controls for your Simple Western™ experiment.
1. A no lysate control. This includes the p...
Can your antibodies be used in automated western blotting systems?
Yes, many of our primary antibodies can be used for Simple Western™ on the ProteinSimple Jess™ instrument. We are continually testing our products on this instrument and are add...
What lysis buffers are compatible with the ProteinSimple system?
Bio-Techne has tested various commercial buffers and common buffer components for compatibility with Simple Western™ and has found that some buffer components may negatively affect...
What is the best titration range to test in order to determine the optimal antibody dilution when using the ProteinSimple system?
When we validate an antibody for Simple Western™ we titrate the antibody dilution against a broad range of lysate concentrations. We recommend testing at least three antibody dilut...
Can I use tissue extracts with the ProteinSimple Jess™ system?
Tissue extracts are appropriate to use as long as they are prepared in buffers that are compatible with Simple Western™. Tissue extracts prepared in 1X SDS buffer (Blue Loading Buf...