Can I change the final concentration of BrdU when using the BrdU Cell Proliferation Assay Kit?

We have not tested changing the final concentration of BrdU while using the BrdU Cell Proliferation Assay Kit. BrdU was originally designed as a chemotherapeutic agent and lethal levels can evoke a DNA damage response, so if the concentration is too high, we are not confident the cells would be healthy enough to proliferate.

Last updated: September 12, 2024