Can I immunostain or perform cell hashing with conjugated antibodies from another company before an InTraSeq™ experiment?

While it is possible to stain live cells with conjugated antibodies or hashing reagents before an InTraSeq experiment, it is not recommended. Since the binding properties of antibodies may perform differently when using InTraSeq reagents, we highly discourage using non-InTraSeq validated 3’ conjugates in Step C. Immunostaining (Day 2).

If performing an immunostaining step prior to the InTraSeq 3’ protocol, we highly recommend beginning the immunostaining with at least 2 million cells to guarantee an adequate number of cells in Step 1.

Last updated: September 18, 2024