Render Target: SSR
Render Timestamp: 2024-12-06T16:09:37.761Z
Commit: 224419269841c11382c4555dbee545259bf6c379
Cell Signaling Technology Logo
1% for the planet logo
< Back to Support Article Search Results

Can I isolate and fractionate RNA with the Cell Fractionation Kit #9038?

We have not attempted RNA isolation using the Cell Fractionation Kit #9038, as this is not the intended application. The buffers and protocol (centrifugation times, speed, temp, etc.) have not been optimized for RNA and none of the buffers are RNase-free. In addition, for RNA isolation, cell pellets need to be subsequently treated with TriZol, chloroform, and isopropanol. Even if the fractionation buffers were RNase-free, it is difficult to speculate if any components could possibly interfere with RNA isolation. RNA isolation is sensitive and can be tricky, therefore I would not recommend complicating the RNA extraction/isolation process by simultaneously fractionating cells for protein. There are separate RNA Subcellular Isolation Kits commercially available.

Last updated: September 12, 2024

Was this article helpful?

Technical Support

Email: [email protected]

Send Us a Message

Call: 877-678-8324

Customer Support

Email: [email protected]

Send Us a Message

Call: 877-616-2355

Fax: 866-432-6112

Contact Sales

Email: [email protected]

Send Us a Message

Call: 877-616-2355