Can I reuse PTMScan® antibody beads?

The “classic” PTMScan® kits that use agarose beads and the PTMScan HS kits that use magnetic beads are both designed for a single PTM peptide enrichment. The nature of the acidic elution step, which leads to the release of bound PTM peptides, denatures the antibodies. This denaturing step alters the conformation of the antibodies. Therefore, employing these antibodies for subsequent enrichments may produce inconsistent results in PTM peptide enrichment.
 

Last updated: August 25, 2023