Can I scale to a 96-well plate format using your Senescence β-Galactosidase Staining Kit #9860?

When using the Senescence β-Galactosidase Staining Kit #9860, we regularly scale up and down the amount of buffer used. If you are working with a 96 well plate, we recommend adding more buffer than 50ul per well. As long as the staining solution is made with the same concentration of reagents, adding more buffer should not give you a false positive or negative (assuming the pH of the final staining solution is within the acceptable range of 5.9-6.1). A volume between 200-250uL should be appropriate for a 96 well plate format.

Last updated: February 27, 2024