Can I store my plates in PBS prior to staining with the Senescence β-Galactosidase Staining Kit #9860?

We typically perform this assay using the Senescence β-Galactosidase Staining Kit (#9860) from start to finish in one day and have not paused between fixing and staining. Therefore, we cannot guarantee the results will not be negatively impacted if cells are incubated in PBS buffer after fixing instead of immediately proceeding to overnight staining. However, we have spoken with numerous customers who have paused after fixing, stored their cells in PBS at 4C for a few days prior to staining and have had great success.

Last updated: September 12, 2024