Can I test my fractionated samples from the Cell Fractionation Kit #9038 kit via mass spectrometry?

Our Cell Fractionation Kit #9038 provides a fast and efficient way of separating cultured cells into three distinct fractions for analysis by SDS-PAGE and western blotting. We have not tested fractionated samples using mass spectrometry in-house and it is difficult to speculate how well they would work. The buffer contains SDS so you may need to use a commercially available SDS purification kit if you would like to prepare the fractionated samples for mass spectrometry by in-solution digestion; in-gel digestion would account for SDS removal in the extraction already. We cannot guarantee activity by mass spectrometry as it is not the primary intended use of this kit.

Last updated: September 12, 2024