Can I use primers from the Illumina Nextera Library preparation system or design my own primers to amplify the DNA library generated from the CUT&Tag Assay Kit #77552??

Yes, you can use primers from the Illumina Nextera Library preparation system or self-designed primers to amplify the DNA library generated from the CUT&Tag DNA using the CUT&Tag Assay Kit #77552. We recommend comparing your sequences with the CST® primer sequences on pages four and five of the CUT&Tag Dual Index Primers and PCR Master Mix for Illumina Systems #47415 product datasheet. It's important to ensure that the primers you use are compatible with the sequences you are amplifying and the downstream applications, such as sequencing on the Illumina platform. If you are using non-CST CUT&Tag Index primers, we suggest using our non-hot start polymerase CUT&Tag PCR Master Mix #63228 for efficient amplification of your CUT&Tag DNA library. 
 

Last updated: September 12, 2024