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Can I use PTMScan® LysC Protease #84748 for tandem mass tag (TMT) labeling?

We have used LysC in conjunction with trypsin for TMT-labeling-based studies to reduce miscleavages. For TMT-based workflows, we recommend a higher LysC:substrate ratio of 1:100 compared to the 1:250 ratio used in PTMScan experiements.

Last updated: May 8, 2024

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