Can I use your Phosphate Buffered Saline with Tween® 20 (PBST-20X) #9809 to make my primary, blocking, and secondary buffers for western blotting?

Our in-house testing indicates that using Phosphate Buffered Saline with Tween® 20 (PBST-20X) #9809 as the base buffer for washes, blocking, and antibody incubation can interfere with the signal of some of our antibodies. Therefore, we do not recommend using this buffer for western blotting with our antibodies. Instead, we recommend using Tris Buffered Saline with Tween® 20 (TBST-10X) #9997. 

For further guidance on setting up a successful western blot, please see our Western Blot Experimental Design Tips.
 

Last updated: September 12, 2024