Can my CST antibody be used for fluorescent western blot?

We do not routinely validate our products for use in fluorescent western blot; however, many are expected to work well in this application with a few protocol modifications. When performing fluorescent western blot, we recommend omitting Tween-20 from the blocking step as well as allowing the membrane to dry completely prior to imaging to achieve optimal results. Our recommended protocol for fluorescent western blotting can be found here. 

Last updated: September 12, 2024