Can the Cell Fractionation Kit #9038 be used for frozen cell pellets?

We have had customers perform this assay using frozen cell pellets, prior to adding any fractionation buffer, and the subsequent fractionation worked fine. Once you start fractionating, we recommend following the protocol through to the end. We do not recommend leaving the cell pellets frozen for a prolonged period of time because, even frozen, proteases and phosphatases can slowly degrade the sample. However, leaving the frozen pellet over a weekend or a few days should not greatly affect results. Results may vary depending on the stability of your specific targets of interest. 

Last updated: September 12, 2024