Can the Phospho-β-Catenin (Ser33/37) Antibody #2009 bind if the sites are singly phosphorylated or is the phosphorylation at both sites necessary?

We have tested the reactivity of the Phospho-β-Catenin (Ser33/37) Antibody #2009 to Ser33/37 by peptide dot blot. This antibody showed strong signal when phosphorylated at Ser37 alone and when phosphorylated at both Ser33 and Ser37. There was weak signal when the peptide was phosphorylated at Ser33 alone, suggesting that the Ser37 contributes more strongly to antibody binding compared to Ser33. No signal was seen when these sites were not phosphorylated. It is our understanding that in vivo the sites (S33/S37/T41) in this region are progressively phosphorylated as a cassette so individual phosphorylated sites would likely be rare in vivo.

Last updated: September 12, 2024