Technical Support Articles
Results (694)
Can the Lambda Protein Phosphatase Kit #89726 be stored at -20°C?
The Lambda Protein Phosphatase #56206 included in the Lambda Protein Phosphatase Kit #89726 is temperature sensitive. Therefore, we recommend storing the kit at -80°C. However, the kit is stable at -2...
Are any of your SARS-CoV-2 Spike Proteins suitable for cell culture?
Unfortunately, we have not tested our SARS-CoV-2 Spike Proteins in cell culture. Additionally, these products are not sterile, therefore we would not recommend using them in this manner.
Should I be concerned that my Concanavalin A beads "clump together" in some of my CUT&Tag experiments?
Concanavalin A beads can clump together rather easily, especially when cells become lysed. Make sure your cells are healthy and be sure to treat them very gently during the cell wash. We also recommen...
How were the sequences of the PTMScan® Control peptides chosen?
The sequences of the synthetic peptides were based on modified peptides that we have consistently recovered and identified in human and mouse samples during our inte...
What secondary antibodies do you recommend for fluorescent western blotting?
We currently have four different secondary antibodies approved for use in fluorescent western blotting, depending on species and wavelength of detection:
- Anti-mouse IgG (H+L) (DyLight™ 680 Con...
What is the benefit of carrier vs. carrier free cytokines?
Our cytokines and growth factors are lyophilized and supplied either with 20ug BSA per 1ug of cytokine (with carrier) or without added protein (carrier free). BSA minimizes adsorption to surfaces and ...
Why do I see a doublet with my beta-Actin antibody?
It is not uncommon to see a doublet with our beta-Actin antibodies depending on the samples being tested. It is likely caused either by phosphorylation of beta-Actin on its many phosphorylation sites,...
Can I use the Anti-rabbit IgG, HRP-linked Antibody #7074 for IHC?
We have not validated the Anti-rabbit IgG, HRP-linked Antibody #7074 for IHC in-house. Therefore, it is not recommended for this application. We do, however, recommend our SignalStain Boost IHC Detect...
Which isotype control should I use for IHC?
For mouse IgGs, you will need to determine the isotype of the primary antibody and use the appropriate isotype control:
IgG1: Mouse (G3A1) mAb IgG1 Isotype Control #5415
IgG2a: Mouse (E5Y6Q) mAb...
Why is it important to include both positive and negative controls in your IF experiment?
Positive and negative controls are at the heart of any good experiment. Without them, it is difficult to assess root causes when troubleshooting. Positive controls should be included to demonstrate: <...
What is the formulation of Hematoxylin #14166?
The Hematoxylin #14166 formula is based on Gill's formulation however, this formulation does not contain alcohol or mercury.
Do I need to optimize the Tn5 tagmentation conditions for different cell types when performing CUT&Tag?
Our optimized tagmentation conditions for the CUT&Tag assay work well across multiple cell lines, both adherent and suspension. We recommend using 2 μL of CUT&Tag pAG-Tn5 (Loaded) #79561 in...
Is the “Best By” date the expiration date for the product? Will the product still work after that date?
Our products can be expected to perform at optimal levels up until the printed "Best" by date on the vial when stored under the recommended conditions. Many of our products will work well af...
Do you have a methylene blue dot blot staining protocol?
We have found that the methylene blue staining of the amounts of DNA spotted when performing a DNA dot blot can be tricky and may need optimization.
1. It is very important to perform the methy...
Can I combine antibodies used in a SignalStar™ assay with direct conjugates?
SignalStar Multiplex IHC kits and reagents have been validated for use in combination with direct conjugates. The SignalStar™ Fluorescence Removal Kit Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic detergent used as a cell p... We have several total Akt antibodies that are validated for IF-IC with mouse samples. These are:
Why do I need to use the three buffers in the Cell Fractionation Kit #9038?
Which total Akt antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) with mouse samples?
How should I resuspend and aliquot the PTMScan® LysC Protease #84748?
To resuspend the PTMScan® LysC Protease #84748, we recommend allowing the vial to equilibrate at room temperature for a few minutes after removing from the -20C freezer. Next, spin the vial at 1,0...
Can I use PTMScan® LysC Protease #84748 for tandem mass tag (TMT) labeling?
We have used LysC in conjunction with trypsin for TMT-labeling-based studies to reduce miscleavages. For TMT-based workflows, we recommend a higher LysC:substrate ratio of 1:100 compared to the 1:250 ...
What is the solution formulation of the PTMScan Control peptides?
The solution formulation of the PTMScan Control peptides is 25% acetonitrile, 74.9% water, 0.1% trifluoroacetic acid (V:V).
Can CUT&RUN enriched DNA be analyzed by qPCR, or do I have to do NGseq to analyze my data?
Our CUT&RUN Assay Kit #86652 is compatible with downstream qPCR analysis. The pr...
Which phospho-AMPKα antibody do you suggest for my western blot experiment?
The selection of a phospho-AMPKα antibody depends on the subunit of interest. Cell Signaling Technology has four antibodies that target phospho-AMPKα:
- Phospho-AMPKα (Thr172) (40H9) R...
What is the fewest number of cells I can use in a reaction with the CST CUT&Tag kit?
We have shown that our CUT&Tag Assay Kit #77552 works with as few as 5,000-10,000 cells for histone modifications and 20,000 cells for transcription factors and cofactors.
What is the species reactivity of the SUMO remnant PTMScan® kits that utilize WaLP for peptide digest?
Wild type alpha-lytic protease (WaLP) is a serine endopeptidase that cleaves at the carboxyl terminal side of amino acids alanine, serine, threonine, and valine.
WaLP recognizes and cleaves SUM...
Can I use the Cell Fractionation Kit #9038 with tissue samples?
Since the Cell Fractionation Kit #9038 is primarily meant to be used for cultured cell lines, the buffers have not been optimized for use with tissue samples. Therefore, the fractions may not be compl...
Which isoforms does the ba1/AIF-1 (E4O4W) XP® Rabbit mAb #17198 detect?
Product Iba1/AIF-1 (E4O4W) XP® Rabbit mAb #17198 is predicted to detect isoform 1 and isoform 2 of the Ionized calcium-binding adaptor molecule 1 (Iba1), also known as allograft inflammator...
Will your GFP antibodies detect Turbo GFP?
The GFP Antibody #2555, GFP (4B10) Mouse mAb #2955, GFP (5G4) Mouse mAb #55494, and GFP (D5.1) XP® Rabbit mAb #2956 are not expected to detect Turbo GFP due to low sequence homology.
Will the SignalStain® DAB Substrate Kit #8059 still perform as expected if the material has been frozen?
While the SignalStain® DAB Substrate Kit #8059 does have a recommended storage temperature of 4°C, we have found tha...
What is the expected yield of a CUT&Tag DNA library?
The expected yield of an amplified CUT&Tag DNA library depends on the DNA quantification system used.
- If you are using a NanoDrop Spectrophotometer or QIAxpert System, the typical reading ...
Which species does the Caspase-3 Activity Assay Kit #5723 work in?
We have validated the Caspase-3 Activity Assay Kit #5723 in human and mouse cell lines. However, as long as there is active caspase-3 present, this kit is predicted to work with most cell lines. Depen...