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Can you enrich for more than one PTM in a PTMScan® sample?

Yes, the flow through from a PTMScan experiment can be saved and stored at -80C for future enrichments. We have performed 5 different PTM enrichments sequentially from one sample. The limitation would be the choice of the initial protease used for the digest. For example, a UbiScan PTMScan requires peptides digested with trypsin and the flow through peptides may not be ideal to capture peptides that require an arginine (R) residue in the phospho-motif as in the AKT or AMPK substrate PTMScan kits.

Last updated: February 27, 2024

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