What advice do you have for collecting samples of adherent cell lines versus suspension cell lines for CUT&Tag experiments?

For CUT&Tag assays using adherent cell lines, the first step is to detach the cells from the dish. We recommend using trypsin to detach the cells. Alternatively, Accutase Cell Dissociation Reagent can be used, but the resulting cell pellet doesn't pack as well during centrifugation, and cells can be lost during the wash steps. Suspension cells are easier to manage for CUT&Tag assays. First, count the cells using a hemacytometer or auto-cell counter, then collect the number of cells needed.

In our in-house CUT&Tag experiments, we have not observed a significant difference in assay performance between adherent and suspension cell lines.

Last updated: September 12, 2024