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Do I need to sonicate my genomic DNA for use in MeDIP and dot blot?

If your DNA fragments are larger than 500 base pairs, we recommend sonicating to form 200-500 base pair fragments that will more readily denature. Sonication should be performed prior to starting the MeDIP or dot blot protocol. DNA fragment size can be analyzed by gel electrophoresis on a 1% agarose gel with a 100 bp DNA marker.

Last updated: September 12, 2024

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