Do the recombinant SARS-CoV-2 spike proteins from CST have the same folding as the native SARS-CoV-2 spike protein?

Unfortunately, we cannot confirm that the recombinant SARS-CoV-2 spike proteins we provide are in exactly the same conformation that would be expected in nature, as we do not yet have direct data addressing this issue. That being said, our recombinant SARS-CoV-2 spike proteins are expressed in mammalian cells to try to ensure the presence of secondary (post-translational) modifications, which would not happen in a prokaryotic expression system. Furthermore, we suspect the trimer is closer to native folding than the monomer, but without NMR or X-Ray crystallography, we have not been able to confirm this. 

Last updated: September 12, 2024