Do you have a protocol for lysing in a 96-well plate (no sonication)?

For lysis in 96-well assay plates, we recommend incubation in the provided lysis buffer for ~30 min at 4°C with gentle shaking. While sonication is difficult in small volumes, we strongly recommend running the sample through a fine gauge needle to ensure full membrane shearing. If the target is not nuclear, you may be able to skip this step.

Last updated: September 12, 2024