Do you have any specific advice for working with adherent cell lines in a CUT&RUN assay? How about suspension cell lines?

With adherent cell lines, one first needs to detach the cells from the dish. We recommend using trypsin to detach the cells. Alternatively, you can use accutase, but the resulting cell pellet doesn't pack as well during centrifugation, so you tend to lose cells during the wash steps. Suspension cells are much easier. Just count the cells using a hemacytometer or auto cell counter and then collect the number of cells you need. We don't observe a significant difference in assay performance with adherent versus suspension cells.

Last updated: February 27, 2024