Do you have any tips for using FACS-isolated cells in CUT&RUN experiments?

We have no specific tips for using FACS-isolated cells in CUT&RUN experiments. Like a cultured cell suspension, if your isolated cells are in less than 40ul PBS, you can move directly to Concanavalin A bead binding by adding wash buffer for a total of 100ul per reaction. Otherwise, you need to either spin the cells and remove some of the PBS or add wash buffer and Concanavalin A beads proportionally based on the volume of PBS you have in your sorted cell sample. In our experience, the Concanavalin A beads do not bind to cells in the pure PBS environment.

Last updated: September 12, 2024