Do you have recommendations for p53 western blot multiplex pairs?

We have not conducted any fluorescent western blot multiplex experiments with p53. Unfortunately, many p53 antibodies have antigenic regions that overlap or have not been precisely mapped. For human samples, our Phospho-p53 (Ser15) Antibody #9284 or our Phospho-p53 (Ser15) (E9Y4U) Rabbit mAb #82530 in combination with p53 (DO-7) Mouse mAb #48818 may work, however, the binding epitope of #48818 has not been mapped. Alternatively, our Phospho-p53 (Ser15) (16G8) Mouse mAb #9286 could be tried in combination with our p53 (E9B5W) Rabbit mAb #30313. #30313 is the only total p53 antibody that we know has an epitope that resides outside of the N-terminus in the p53 tetramerization domain.

Last updated: February 27, 2024