Does CUT&RUN work for plant cells?

We have not tested our CUT&RUN Assay Kit using plant cells. However, Xiao-Yu Zheng et al. (2019; PMID30719569) describes a method for pre-treating plant tissues and preparing the nuclei for CUT&RUN. Our kit can be used downstream of this nuclei preparation protocol. In this case, digitonin may not be required for cell permeabilization during the CUT&RUN assay, but it may help further permeabilize the cell membrane if the plant cells are not 100% homogenous.

Last updated: February 27, 2024