Does your Cas9 (7A9-3A3) Mouse mAb #14697 detect the D10A/H840A mutant (dCas9) and the D10A mutant (niCas9)?

Our Cas9 (7A9-3A3) Mouse mAb #14697 was produced with a recombinant protein specific to the amino terminus of Cas9 from Streptococcus pyogenes (UniProt ID #Q99ZW2; https://www.uniprot.org/uniprot/Q99ZW2). The precise epitope has not been mapped. However, this antibody has been shown to detect nuclease-deficient Cas9 (dCas9) protein and Cas9 nickase (niCas9) protein (please see the western blot image on the product datasheet; https://media.cellsignal.com/pdf/14697.pdf). dCas9 is a D10A/H840A mutant and niCas9 is a D10A mutant.

Last updated: April 19, 2024