Does your CUT&RUN Assay Kit work with isolated nuclei?

Our CUT&RUN Assay Kit is optimized for use with whole cells and works with a large number of cell lines, both adherent and suspension. While it is not typically necessary to pre-isolate cell nuclei for CUT&RUN, our kit should work with pre-isolated nuclei because the Concanavalin A beads bind to glycoproteins on the surface of both cells and nuclei. Pre-isolation of cell nuclei should only be necessary for cells that are not efficiently permeabilized by digitonin, such as yeast or plant cells that both contain cell walls.

Last updated: May 5, 2023