For imaging purposes, which caspase-3 antibody do you recommend for use on frozen rodent tissue?

For imaging purposes, we recommend using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb #9664 when working with rodent tissue.  We have noted non-specific labeling in specific sub-types of healthy cells in fixed-frozen mouse tissues (e.g. pancreatic alpha-cells) and nuclear background in rat samples with Cleaved Caspase-3 (Asp175) Antibody #9661 and Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb #9579.

Last updated: October 2, 2023