How do I observe more robust signal when using your Poly/Mono-ADP Ribose (E6F6A) Rabbit mAb #83732?

The pathway induction from H202 treatment may be more robust than the LPS treatment. We have previously observed that buffer and temperature conditions at the lysis stage can affect basal levels of PARylation.  Notably, lysis at RT yielded lower observable PAR than lysis on ice (4 deg C). Also, performing the initial lysis in a PDE assay buffer (available from several vendors) yielded higher observable PAR than lysis directly into SDS loading buffer with DTT.  Lastly, cells overgrown in culture may also show higher basal levels of PAR. 

Last updated: September 12, 2024