How do I prepare the samples shown in the web image for your Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Plate-Based) #23833?

The samples shown in the web image for our Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Plate-Based) #23833 were prepared as follows:
1. Treat cells with 200 nM Doxorubicin for 24 hrs, then aspirate media and replace with fresh growth media. Rinsing with a small amount of the growth media to remove all old media prior to adding the final volume for the rest phase is recommended, but not necessary.
2. Allow the cells to rest for 3 days. The 3 day rest period is necessary as it takes time to build up β-Galactosidase levels in the cells.

Last updated: September 12, 2024