How do you make the 4X RNA Denaturing Buffer for RNA dot blot?

4X RNA Denaturing Buffer Recipe for use with our RNA dot blot protocol:

657ul Formamide @99.5%
210ul of 37% Formaldehyde
133ul of 10x MOPS buffer

Final formulation (considering rounding):
66% Formamide
8% Formaldehyde
1.33X MOPS (10x MOPS, which is made up of 0.2M MOPS, 0.05M Sodium Acetate 0.01M EDTA, 0.01M EGTA)

This is used in the sample preparation section of our RNA dot blot protocol (Section B, Step 1).

Last updated: September 12, 2024