How do you obtain the motif logos for your PTMScan® product datasheets?

We create the motif logos for our PTMScan product datasheets by running a bioinformatics workflow that takes the primary peptide sequence from a PTMScan IAP and extends this -7 and +7 amino acids from the PTM site. This generates a unique list of sequences with the PTM of interest in the 0 position. We then input this list into the SiteLogo tool in PhosphoSitePlus (https://www.phosphosite.org/sequenceLogoAction) to generate the motif logo.

Last updated: February 27, 2024