How does the spike-in DNA allow for normalization of the CUT&RUN assay?

A specificied amount of pre-fragmented yeast genomic DNA is added to each CUT&RUN reaction after the digestion and prior to DNA purification. This spike-in component allows you to normalize for differences in DNA purification, qPCR, library preparation and sequencing efficiencies between samples. The protocol included in our CUT&RUN Assay Kit #86652 provides detailed instructions for the spike-in DNA control for both qPCR and NG-seq. It is important to note that significantly different amounts of spike-in DNA are required for qPCR vs. NG-seq, so please read the protocol carefully.

Last updated: September 12, 2024