How is G6PD activity distinguished from 6PGD activity when using the Glucose-6-Phosphate Dehydrogenase (G6PD) Activity Assay Kit #12581?

Glucose-6-phosphate (G6P), in the presence of NADP, is oxidized by Glucose-6-Phosphate Dehydrogenase (G6PD) to generate 6-phosphogluconolactone and NADPH. The NADPH is then amplified by the diaphorase-cycling system to produce highly fluorescent resorufin molecules. We assume that by measuring the resulting NADPH, the signal is proportional to the G6PD activity in your sample. 6-Phosphogluconate dehydrogenase (6PGD) also uses NADP+ to generate NADPH, and therefore, may contribute to the overall signal. As stated in the Specificity / Sensitivity section on the product webpage, the Glucose-6-Phosphate Dehydrogenase (G6PD) Activity Assay Kit #12581 detects sample G6PD activity, however the presence of NADH and NADPH may interfere with the assay. Therefore, it may be difficult to distinguish between G6PD and 6PGD activity when using this kit.

Last updated: September 12, 2024