How is the PTMScan® HS Ubiquitin/SUMO Remnant Motif (K-ε-GG) Kit #59322 different from traditional, agarose bead PTMScan®?

The PTMScan® HS Ubiquitin/SUMO Remnant Motif (K-ε-GG) Kit #59322 uses the same antibody as in the traditional PTMScan Ubiquitin Remnant Motif (K-ε-GG) Kit #5562 and PTMScan Pilot Ubiquitin Remnant Motif (K-ε-GG) Kit #14482. This means it has the same affinity for the K-e-GG remnant after trypsin cleavage.

However, we have changed the antibody – bead linkage to be near-covalent in strength so the antibody does not contaminate the PTM peptides during the elution step. This prevents issues with the liquid chromatography column clogging and increases the yield of PTM peptides identified. The specificity is also improved with changes to the bind and wash buffers that help reduce non-specific peptide binding.

In addition, the new HS kit beads are magnetic, rather than agarose, which simplifies the workflow by eliminating multiple centrifugation steps during the bead washing and elution steps.

 

Last updated: April 16, 2024