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How much DNA is required to make my library for ChIP-seq?

The typical DNA yields from ChIP experiments range from 0.2-2 ng/µl when targeting transcription factors or cofactors and from 2-20 ng/µl when targeting histone modifications. If the concentration is within this range, then you should be able to continue with ChIP-seq. Our DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795 can generate a library with 0.5ng ChIP-DNA.

Last updated: September 12, 2024

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