How should I resolve RyR1 for western blot detection with the RyR1 (D4E1) Rabbit mAb #8153?

We recommend that you run your samples on a 3-8% Tris-Acetate gel in order to get separation of large proteins. We also suggest that you use a standard wet transfer, transferring at 70V for 5 hours with a 0.2 um nitrocellulose membrane, in a 4C refrigerator. We also recommend reducing the methanol content in your transfer buffer to 5-10%. Using these conditions will ensure optimal and clean transfer of the RyR1 protein from the gel to the membrane.

Last updated: September 12, 2024