How should tissue samples be prepared for the PTMScan® Acetyl-Lysine Motif [Ac-K] Kit #13416?

Tissue samples should be placed in 1 ml of urea lysis buffer per 100 mg of tissue wet weight. For example, a 300mg tissue sample would be homogenized in 3mls of urea lysis buffer. We use a Polytron tissue homogenizer, and homogenize the sample 3 times for 10 seconds, then continue the protocol at section 1, B. 3. and sonicate the homogenate before centrifugation. Please note that the acetyl-lysine antibody will cross react with carbamylated lysine. To avoid this, please make sure that the urea lysis buffer is made fresh, and perform the reduction with DTT at room temperature to reduce any carbamylation.

Last updated: September 12, 2024