Is it possible to perform CUT&RUN on tissue samples like heart, brain, or developing mouse tissues?

Yes. We have shown that our CUT&RUN Assay Kit #86652 works with mouse liver, mouse brain, and mouse heart tissues with as little as 1mg of tissue for each CUT&RUN reaction. Fresh tissue samples work with histone modifications and high abundance DNA binding factors like CTCF. However, for optimal results with transcription factors, cofactors, and difficult tissue types, a light to medium fixation (0.1% formaldehyde, 2-10 min) should be performed and slightly more starting material (2.5mg-5mg) should be used. Fixed tissues can be frozen for up to 6 months before use in the CUT&RUN assay.

Last updated: September 12, 2024