Is it possible to perform CUT&Tag on fixed frozen cells?

Yes. Cell fixation is recommended when the cells are very fragile (easily lysed during experiments), when the cellular signaling pathway is sensitive to the Concanavalin A beads used for cell binding, or when you hope to freeze the cell pellets for future use. If fixation is necessary, refer to the CUT&Tag Assay Kit Protocol, Appendix A to lightly fix cells prior to the CUT&Tag experiment. We find that live cells produce better results than fixed cells in the CUT&Tag assay. Therefore, we recommend using live cells, if possible, unless fixation is necessary for the scenarios mentioned above.

Last updated: February 29, 2024