Is it possible to perform CUT&Tag on samples like heart, brain, or developing mouse tissues?

Yes. We have shown that our CUT&Tag Assay Kit #77552 works with mouse liver, mouse brain, and mouse heart tissues for histone targets with as little as 1 mg of tissue for each CUT&Tag reaction. However, non-histone targets such as transcription factors and cofactors are not well enriched in tissues in the CUT&Tag assay. For analysis of transcription factors and cofactors in tissues, we recommend using the CUT&RUN Assay Kit #86652. Fresh tissues typically generate comparable or stronger CUT&Tag signals than fixed tissues.

If fixation is necessary, refer to the CUT&Tag Assay Kit Protocol, Appendix B: Fixed Tissue Sample Preparation, for the light fixation of tissues prior to the CUT&Tag experiment. Fixed tissues can be frozen for up to six months before use in the CUT&Tag assay.

Last updated: September 12, 2024