Is there a way to counterstain when using the Senescence β-Galactosidase Staining Kit #9860?

We have not tried counterstaining in-house, however, the paper below mentions using Hoechst as a fluorescent DNA dye to quantify the cells:  

Baker, D. J. et al. (2004) BubR1 insufficiency causes early onset of aging-associated phenotypes and infertility in mice. Nat. Genetics 36, 744-749

You may be able to find other references on the web, as this kit is popular and there are many publications citing its use. We use hematoxylin routinely in our IHC experiments, and, consequently, have limited experience with alternatives.

For further suggestions, it may be helpful to seek out information within the Histonet (http://www.histosearch.com/histonet.html). This is a well-monitored discussion forum for histology professionals. There is an archive of queries that can be searched, or a new query can be posted. There may be suggestions or observations using these methods that could prove helpful.

Last updated: February 28, 2024