Is there a way to counterstain when using the Senescence β-Galactosidase Staining Kit #9860?
We have not tried counterstaining in-house, however, the paper below mentions using Hoechst as a fluorescent DNA dye to quantify the cells:
Baker, D. J. et al. (2004) BubR1 insufficiency causes early onset of aging-associated phenotypes and infertility in mice. Nat. Genetics 36, 744-749
You may be able to find other references on the web, as this kit is popular and there are many publications citing its use. We use hematoxylin routinely in our IHC experiments, and, consequently, have limited experience with alternatives.
Last updated: October 15, 2025
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