Must I perform an Fc receptor blocking step with the CD16/CD32 (2.4G2) Rat mAb #88280 in every immunostaining experiment?

It is difficult to predict when the Fc receptor blocking step is necessary, due to the variable interaction of host species with different antibody isotypes and the interference caused by fluorescent dyes directly conjugated to the antibody Fc domain. The presence of these dyes is often sufficient to disrupt Fc receptor binding. If you are unable to conduct an experiment to assess nonspecific binding, we recommend using the CD16/CD32 (2.4G2) Rat mAb #88280 to prevent any unintended antibody binding to the Fc receptor.

Last updated: September 12, 2024