When comparing my SignalStar™ staining to the chromogenic staining on a serial section, I see more positive cells. How do I know if this excess staining is correct?

During optimization, we have found that fluorescent staining may show higher % positivity than chromogenic staining. To ensure that any excess staining is specific, confirm that the correct subcellular localization and co-localization are demonstrated compared with other stains. For example, if all CD8+ cells are CD3+, any excess CD8+ staining compared to the chromogenic staining is likely correct.

Last updated: April 30, 2024