The gel images for your mFc-Tag products show reduced and non-reduced samples. Can you tell me what this means?

These samples represent the non-reduced protein and the denatured protein reduced by DDT. Standard western blotting protocols use denaturing conditions to expose epitopes, therefore the reduced sample more accurately reflects the protein's predicted molecular weight. The non-reduced sample shows the observed molecular weight of the non-denatured, folded protein. The difference in the band size of the reduced and non-reduced samples is due to the secondary structure of the protein affecting the protein's mobility in a gel. 

Last updated: February 27, 2024