What can I use as a loading control with the Cell Fractionation Kit #9038?

Due to the nature of the Cell Fractionation Kit #9038 and the localization of each of the proteins in the Loading Control Sampler Kit #5142, the proteins will not be equally expressed in all of the fractions. We have not found a target that shows equal expression across all the cytoplasmic and membrane fractions. The whole cell lysate (WCL) can be used as a reference to compare against each fraction. Efficiency of separation can be measured using the antibodies in the Cell Fractionation Antibody Sampler Kit #11843.

Last updated: September 12, 2024