What controls can I use to show my CUT&RUN experiment is working?

Whether performing CUT&RUN with downstream qPCR or NG-seq analysis, we always recommend using a CUT&RUN-validated positive control antibody like our Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751 and a negative control antibody like our Rabbit (DA1E) mAb IgG XP^® Isotype Control Antibody #66362. These antibodies can be paired with our SimpleChIP^® Human RPL30 Exon 3 Primers #7014 or SimpleChIP^® Mouse RPL30 Intron 2 Primers #7015 to show that your CUT&RUN assay is working regardless of the performance of your desired target-specific antibody. All of these controls are included in our CUT&RUN Assay Kit #86652.

Last updated: September 12, 2024