What controls should I include when performing a Simple Western™ experiment?

In addition to a known positive control sample, we recommend the following negative controls for your Simple Western™ experiment.

1. A no lysate control. This includes the primary antibody at the highest concentration tested with samples containing only 0.1X sample buffer + Fluorescent Master Mix (FMM). This control will determine if there is any cross-reactivity between the primary antibody and the internal standards used in the assay.

2. A no primary antibody control. This includes the antibody diluent (without antibody) plus the lysate used at the highest concentration. This control will help to rule out any cross-reactivity between the secondary antibody and the lysate.

Last updated: April 13, 2023