What if the entire CUT&Tag kit is stored at -20℃ or left at room temperature upon receipt?

The CUT&Tag Assay Kit #77552 contains components that are packaged carefully and need to be stored at either room temperature (RT), 4℃, or -20℃. It's important to store each reagent at the appropriate temperature to ensure optimal functionality.

However, if the entire kit is accidentally stored at -20℃, the Concanavalin A beads will lose some activity. If this happens, we recommend purchasing our standalone Concanavalin A Magnetic Beads and Activation Buffer #93569 for future experiments, especially when working with low abundance and/or weak DNA binding proteins. In addition, to preserve proper performance, thaw the DNA spin columns slowly (i.e., thaw at 4℃ first, then calibrate to room temperature) and make sure there is no condensation inside the column before use. If needed, DNA Purification Buffers and Spin Columns (ChIP, CUT&RUN, CUT&Tag) #14209 are also available for purchase as a standalone product. All of the other reagents in the kit are acceptable for use after thawing.

If the whole kit is left at room temperature for less than a week, the majority of the reagents will still be good to use, including the pAG-Tn5 (Loaded) enzyme and the Concanavalin A beads. The exceptions are the Spermidine solution and Protease Inhibitor Cocktail, which may have impaired performance. Both the 100X Spermidine #27287 and Protease Inhibitor Cocktail (200X) #7012 are available as standalone products.

Last updated: September 12, 2024